| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL1.2[NlucP] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3233 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pNL1.2[NlucP]载体图谱](/uploads/载体图谱/pNL1.2[NlucP]载体图谱.png)
载体简介
载体序列
LOCUS pNL1.2[NlucP] 3233 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Promoterless vector encoding destabilized NanoLuc(R) luciferase for
measuring the activity of promoter and enhancer sequences.
ACCESSION JQ437371
VERSION .
KEYWORDS pNL1.2[NlucP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3233)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Wednesday, February 26, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3233
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..612
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
CDS 616..735
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 787..908
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1327..1915)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2115..2975)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 3080..3128
/note="synthetic polyadenylation signal"
misc_feature 3142..3233
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggtcttcac actcgaagat
121 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
181 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
241 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
301 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
361 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
421 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
481 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
541 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
601 cgcattctgg cgaattctca cggctttccg cctgaggttg aagagcaagc cgccggtaca
661 ttgcctatgt cctgcgcaca agaaagcggt atggaccggc acccagccgc ttgtgcttca
721 gctcgcatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
781 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
841 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
901 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
961 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
1021 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
1081 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
1141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
1261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
1321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1741 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
1801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
1921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
1981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
2101 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
2161 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
2221 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
2281 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
2341 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
2401 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
2461 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
2521 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
2581 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
2641 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
2701 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
2761 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
2821 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct
2881 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag
2941 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga
3001 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg
3061 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
3121 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
3181 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//
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